Journal: Nature Communications
Article Title: Computationally-designed aptamers targeting RAD51-BRCA2 interaction impair homologous recombination and induce synthetic lethality
doi: 10.1038/s41467-025-66694-9
Figure Lengend Snippet: A High-resolution immunofluorescence images of a single BxPC-3 cell untreated or treated with increasing concentrations of Apt1 in combination with cisplatin. Nuclei are stained with DAPI (blue); RAD51 is shown in green; BRCA2 in red. Scale bar: 10 μm. B Bar graphs illustrating the image-based quantification of a cell population nuclear fluorescence, showing RAD51 (orange) and BRCA2 (blue) under DNA damage conditions and increasing concentrations of Apt1. Experiments were performed in biological triplicates, and data were collected from approximately 500 cells per condition. Source data are provided as a Source Data file.
Article Snippet: After washing, cells were incubated with a mouse anti-BRCA2 primary antibody (Santa Cruz Biotechnology, sc-518154), diluted 1:250 in 5% BSA/PBS, for 1 h at room temperature.
Techniques: Immunofluorescence, Staining, Fluorescence